1. Nitric Oxide - This is a signaling molecule that is paracrine in type. It crosses the plasma membrane by binding to a receptor and affects enzyme activity. Just like the G protein, nitric oxide targets the enzyme, guanylyl cyclase, and thus, makes cyclic GMP. In the body, it affects smooth muscles and blood vessels.
2. Eicosanoids -These are lipid signaling molecules that include prostaglandins, prostacyclin, thromboxanes, and leukotrienes. They break down rapidly.
3. Ligand-gated channel - Unlike the voltage-gated channel, these channels do not open based on voltage, but they are activated or opened because of some signaling molecule (ligand).
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